Presentation Abstract

Student's Name: Laia Llorente '26

Co-registrant Names:

Type of Presentation: Poster Exhibit: One or more registrants displaying posters for discussion

Presentation Title: "Analysis of Caffeine metabolism gene"

Abstract:

The CYP1A2 gene encodes the primary enzyme responsible for metabolizing caffeine, and variation at the rs762551 SNP affects how quickly caffeine is processed. Fast metabolizers (AA) break down caffeine rapidly, while slow metabolizers (AC or CC) experience longer-lasting effects. The purpose of this project is to determine each participant’s CYP1A2 rs762551 genotype using PCR-RFLP analysis and investigate how genetic variation correlates with caffeine consumption habits and physiological responses. Participants collected buccal cells to isolate genomic DNA, amplified the SNP region via PCR, and performed RFLP analysis with Apal and Sacl restriction enzymes.

To connect genotype with caffeine behavior, participants completed a survey assessing frequency and type of caffeine consumption, typical daily intake, perceived effects, duration of effects, and impact on sleep. By comparing survey results with genotypes, students can explore how genetics influences caffeine metabolism, tolerance, and sensitivity, demonstrating the role of genetic variation in individual physiological responses.

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